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💸 Grant Assist
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To develop or improve existing strategies, technologies or methods, or data use strategies to support One Health AMR surveillance. Within this topic projects may: * Develop new or improve existing strategies, technologies, and methods for the detection, analysis, monitoring and use of AMR and AMU data. This can include the analysis of existing data or the application of existing surveillance strategies, technologies, and methods to additional OH settings. * Explore the standardisation, FAIRification and linkage of methodologies, datasets and relevant indicators to perform globally comparative, integrated and triangulated surveillance of AMR/AMU in humans, animals (including companion animals, livestock and wildlife), plants, food, and the environment. The following sub-topics are out of the scope of the call: * antiviral and antiparasitic agents, * proposals solely aiming to extend existing surveillance networks (e.g. GLASS, national surveillance programmes).
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Project plan, ideas, previous grants, etc.
Gene cassettes and cassette arrays in mobile resistance integrons Gene cassettes are small mobile elements, consisting of little more than a single gene and recombination site, which are captured by larger elements called integrons. Several cassettes may be inserted into the same integron forming a tandem array. The discovery of integrons in the chromosome of many species has led to the identification of thousands of gene cassettes, mostly of unknown function, while integrons associated with transposons and plasmids carry mainly antibiotic resistance genes and constitute an important means of spreading resistance. An updated compilation of gene cassettes found in sequences of such ‘mobile resistance integrons’ in GenBank was facilitated by a specially developed automated annotation system. At least 130 different (<98% identical) cassettes that carry known or predicted antibiotic resistance genes were identified, along with many cassettes of unknown function. We list exemplar GenBank accession numbers for each and address some nomenclature issues. Various modifications to cassettes, some of which may be useful in tracking cassette epidemiology, are also described. Despite potential biases in the GenBank dataset, preliminary analysis of cassette distribution suggests interesting differences between cassettes and may provide useful information to direct more systematic studies.
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outline a plan for a project proposal targeted toward this grant
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